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Electrospray ionization ion‐trap multiple‐stage mass spectrometry of Quillaja saponins
Author(s) -
Bankefors Johan,
Broberg Susanna,
Nord Lars I.,
Kenne Lennart
Publication year - 2011
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1935
Subject(s) - chemistry , saponin , electrospray ionization , chromatography , ion trap , mass spectrometry , electrospray , mass spectrum , tandem mass spectrometry , analytical chemistry (journal) , medicine , alternative medicine , pathology
Fifteen identified C‐18 fatty acyl‐containing saponin structures from Quillaja saponaria Molina have been investigated by electrospray ionization ion‐trap multiple‐stage mass spectrometry (ESI‐IT‐MS n ) in positive ion mode. Their MS 1 –MS 3 spectra were analyzed and ions corresponding to useful fragments, important for the structural identification of Quillaja saponins, were recognized. A few key fragments could describe the structural variations in the C‐3 and the C‐28 oligosaccharides of the Quillaja saponins. A flowchart involving a stepwise procedure based on key fragments from the MS 1 –MS 3 spectra of these saponins, together with key fragments from these saponins and 13 previously investigated saponins, was constructed for the identification of structural elements in Quillaja saponins. Peak intensity ratios in MS 3 spectra were found to be correlated to structural features of the investigated saponins and is therefore of value for the identification of regioisomers. Copyright © 2011 John Wiley & Sons, Ltd.