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Ultraviolet photodissociation of chromophore‐labeled oligosaccharides via reductive amination and hydrazide conjugation
Author(s) -
Ko Byoung Joon,
Brodbelt Jennifer S.
Publication year - 2011
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1901
Subject(s) - chemistry , reductive amination , chromophore , photodissociation , hydrazide , amination , photochemistry , ultraviolet , ultraviolet radiation , combinatorial chemistry , organic chemistry , catalysis , radiochemistry , physics , quantum mechanics
The fragmentation patterns of hydrazide‐conjugated and reductively aminated oligosaccharides, including lacto‐ N ‐fucopentaoses and lacto‐ N ‐difucohexaoses, produced on collisionally induced dissociation (CID) and ultraviolet photodissociation (UVPD) in a quadrupole ion trap are presented. The two derivatization methods generate different cross‐ring cleavages on UVPD and CID. UVPD of hydrazide‐conjugated oligosaccharides yield predominant 2, 4 A‐type cross‐ring cleavage ions. In contrast, UVPD of aminated oligosaccharides results mainly in 0, 1 A‐type ions. Moreover, more extensive dual‐cleavage pathways (i.e. internal fragment ions) were observed on UVPD. Copyright © 2011 John Wiley & Sons, Ltd.
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