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Fragmentation pathways of metopimazine and its metabolite using ESI‐MS n , HR‐MS and H/D exchange
Author(s) -
HubertRoux Marie,
Bounoure Frédéric,
Skiba Mohamed,
Bozec Philippe,
Churlaud Florence,
Lange Catherine M.
Publication year - 2010
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1790
Subject(s) - chemistry , fragmentation (computing) , mass spectrometry , electrospray ionization , electrospray , metabolite , ion , analytical chemistry (journal) , chromatography , stereochemistry , organic chemistry , computer science , biochemistry , operating system
Abstract Metopimazine (MPZ) is a phenothiazine derivative used to prevent emesis during chemotherapy where few structural analysis of the aforementioned compound have been described in the literature. Thus, this work reports, for the first time, the detailed study of fragmentation pathways of MPZ and its metabolite (AMPZ) using electrospray ionization (EI) with multistage mass spectrometry (ESI‐MS n ) in positive‐ion mode. The structures of 21 product ions were identified and their accurate masses were determined using high resolution mass spectrometry (HRMS) experiments. Characteristic product ions of these two phenothiazine derivatives are more particularly displayed along with differences between their relative abundances and their structures checked by H/D exchange experiments. Copyright © 2010 John Wiley & Sons, Ltd.