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Selective cleavage of protonated penetratin and its substitutes under low‐energy collision‐induced dissociation condition
Author(s) -
Peng Juan,
Zu Lily,
Fang Weihai,
Huang Lingyun,
Wang Yaru,
He Dacheng
Publication year - 2010
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1748
Subject(s) - chemistry , dissociation (chemistry) , fragmentation (computing) , collision induced dissociation , protonation , mass spectrometry , stereochemistry , tandem mass spectrometry , combinatorial chemistry , organic chemistry , chromatography , ion , computer science , operating system
An understanding of the dissociation of penetratin is important for improving its metabolic stability and cargo‐delivery efficiency. In this study, we describe the selective cleavage of the K15–K16 amide bond of penetratin under the low‐energy collision‐induced dissociation condition in mass spectrometry. A variety of penetratin substitutes have been studied in which key basic amino acids have been substituted within the sequence. The calculated structure indicates that an α‐helix structure prevents the fragmentation of the central peptide domain and the side chain of lysine is involved in the proton translocation process. Copyright © 2010 John Wiley & Sons, Ltd.