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The assay of pterostilbene in spiked matrices by liquid chromatography tandem mass spectrometry and isotope dilution method
Author(s) -
Mazzotti Fabio,
Di Donna Leonardo,
Benabdelkamel Hicham,
Gabriele Bartolo,
Napoli Anna,
Sindona Giovanni
Publication year - 2010
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1718
Subject(s) - chemistry , chromatography , isotope dilution , detection limit , mass spectrometry , tandem mass spectrometry , pterostilbene , electrospray , liquid chromatography–mass spectrometry , electrospray ionization , resveratrol , biochemistry
Pterostilbene ( trans ‐3,5‐dimethoxy‐4‐hydroxystilbene) is an active component found in several plant species, exhibiting important pharmacological properties. A new and reliable method of assaying this phyto compound in various matrices is presented; the assay is based on (1) the selectivity of liquid chromatography (LC) hyphenated with electrospray ionisation (ESI), (2) the specificity of a two‐step mass spectrometric analysis (MS/MS) and (3) the accuracy of the isotope dilution method. The labelled analogue may be conveniently synthesised in a few steps. The sensitivity of the method is confirmed by the very low limit of detection (LOD) and limit of quantitation (LOQ) values achieved in the assay of pterostilbene in two distinct fortified matrices, and is further supported by the observed accuracy values. Copyright © 2010 John Wiley & Sons, Ltd.