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Laser desorption postionization for imaging MS of biological material
Author(s) -
Akhmetov Artem,
Moore Jerry F.,
Gasper Gerald L.,
Koin Peter J.,
Hanley Luke
Publication year - 2010
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1716
Subject(s) - chemistry , ionization , mass spectrometry , laser , photoionization , atmospheric pressure laser ionization , matrix assisted laser desorption electrospray ionization , mass spectrometry imaging , maldi imaging , ultraviolet , desorption , matrix assisted laser desorption/ionization , analytical chemistry (journal) , optics , electron ionization , ion , physics , chromatography , organic chemistry , adsorption
Abstract Vacuum ultraviolet single photon ionization (VUV SPI) is a soft ionization technique that has the potential to address many of the limitations of matrix‐assisted laser desorption/ionization (MALDI) for imaging MS. Laser desorption postionization (LDPI) uses VUV SPI for postionization and is experimentally analogous to a MALDI instrument with the addition of a pulsed VUV light source. This review discusses progress in LDPI‐MS over the last decade, with an emphasis on imaging MS of bacterial biofilms, analytes whose high salt environment make them particularly resistant to imaging by MALDI‐MS. This review first considers fundamental aspects of VUV SPI including ionization mechanisms, cross sections, quantum yields of ionization, dissociation and potential mass limits. The most common sources of pulsed VUV radiation are then described along with a newly constructed LDPI‐MS instrument with imaging capabilities. Next, the detection and imaging of small molecules within intact biofilms is demonstrated by LDPI‐MS using 7.87 eV (157.6 nm) VUV photons from a molecular fluorine excimer laser, followed by the use of aromatic tags for detection of selected species within the biofilm. The final section considers the future prospects for imaging intact biological samples by LDPI‐MS. Copyright © 2010 John Wiley & Sons, Ltd.