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Structural determination of glucosylceramides isolated from marine sponge by fast atom bombardment collision‐induced dissociation linked scan at constant B/E
Author(s) -
Ahn Young Min,
Lee WonWoong,
Jung Jee H.,
Lee SangGi,
Hong Jongki
Publication year - 2009
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1678
Subject(s) - chemistry , fast atom bombardment , moiety , protonation , dissociation (chemistry) , mass spectrometry , collision induced dissociation , fragmentation (computing) , ion , molecule , mass spectrum , stereochemistry , crystallography , analytical chemistry (journal) , chromatography , tandem mass spectrometry , organic chemistry , computer science , operating system
Five glucosylceramides (GlcCers) were isolated by reversed phase high‐performance liquid chromatography from the MeOH extracts of a marine sponge, Haliclona ( Reniera ) sp., collected from the coast of Ulleung Island, Korea, and analyzed by fast atom bombardment mass spectrometry (FAB–MS) in positive‐ion mode. FAB‐mass spectra of these compounds included protonated molecules [M + H] + and abundant sodiated molecules [M + Na] + from a mixture of m ‐NBA and NaI. The structures of these GlcCers, which were similar, were elucidated by FAB‐linked scan at constant B/E. To find diagnostic ions for their characterization, the GlcCers were analyzed by collision‐induced dissociation (CID) linked scan at constant B/E. The CID‐linked scan at constant B/E of [M + H] + and [M + Na] + precursor ions resulted in the formation of numerous characteristic product ions via a series of dissociative processes. The product ions formed by charge‐remote fragmentation provided important information for the characterization of the fatty N ‐acyl chain moiety and the sphingoid base, commonly referred to as the long‐chain base. The product ions at m / z 203 and 502 were diagnostic for the presence of a sodiated sugar ring and β‐ D ‐glucosylsphinganine, respectively. For further confirmation of the structure of the fatty N ‐acyl chain moiety in each GlcCer, fatty acid methyl esters were obtained from the five GlcCers by methanolysis and analyzed by FAB–MS in positive‐ion mode. On the basis of these dissociation patterns, the structures of the five GlcCers from marine sponge were elucidated. In addition, the accurate mass measurement was performed to obtain the elemental composition of the GlcCers isolated from marine sponge. Copyright © 2009 John Wiley & Sons, Ltd.