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Sieve‐based device for MALDI sample preparation. II. Instrumental parameterization
Author(s) -
Cristoni Simone,
Molin Laura,
Crotti Sara,
Rossi Bernardi Luigi,
Seraglia Roberta,
Traldi Pietro
Publication year - 2009
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1637
Subject(s) - chemistry , sieve (category theory) , sample preparation , analytical chemistry (journal) , maldi imaging , matrix (chemical analysis) , sprayer , chromatography , mass spectrometry , matrix assisted laser desorption/ionization , analyte , molecular sieve , desorption , adsorption , organic chemistry , mathematics , geotechnical engineering , combinatorics , engineering
A simple device effective for matrix‐assisted laser desorption/ionization (MALDI) sample preparation, based on the spraying of matrix/sample solution through a stainless steel sieve (sieve‐based device, SBD), has been employed for the preparation of MALDI samples of peptides, polysaccharides and high molecular weight proteins. The instrumental parameters, mainly the sprayer voltage, solution and auxiliary gas flows, and distances between sprayer–sieve and sieve–MALDI sample holder have been optimized. The MALDI spectra obtained by laser irradiation of the microspots so obtained, exhibit resolution and sensitivity higher than those achieved by the commonly employed dried droplets (DD) method. Worth noting is the fact that, in contrast to what is observed in the DD method, identical spectra are obtained by irradiation of different areas of the MALDI sample, showing that by using the SBD the matrix/analyte ratio is constant over the entire surface. Copyright © 2009 John Wiley & Sons, Ltd.