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Surface‐activated chemical ionization and cation exchange chromatography for the analysis of enterotoxin A
Author(s) -
Cristoni Simone,
Zingaro Lorenzo,
Canton Cristina,
Cremonesi Paola,
Castiglioni Bianca,
Morandi Stefano,
Brasca Milena,
Luzzana Massimo,
Battaglia Cristina
Publication year - 2009
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1636
Subject(s) - chemistry , chromatography , electrospray ionization , detection limit , mass spectrometry , chemical ionization , electrospray , ionization , matrix (chemical analysis) , ion suppression in liquid chromatography–mass spectrometry , analytical chemistry (journal) , ion , liquid chromatography–mass spectrometry , organic chemistry
Surface‐activated chemical ionization (SACI) has been widely used in recent years for the analysis of different compounds (e.g. peptides, street drugs, amino acids). The main benefits of this technology are its high sensitivity and its effectiveness under different chromatographic conditions [i.e. ion exchange chromatography and reversed‐phase (RP) chromatography]. Here we used SACI in conjunction with quadrupole time‐of‐flight mass spectrometry to analyze enterotoxin A, which is produced by Staphylococcus aureus , in milk matrix using both RP and ion exchange chromatographies. SACI had increased sensitivity as compared with electrospray ionization. Moreover, the higher quantitation efficiency of this technique, mainly in terms of limit of detection (0.01 ng/ml), limit of quantitation (0.05 ng/ml), linearity range (0.05–50 ng/ml), matrix effect, accuracy (intraday and interday accuracy errors were 9.2% and 10.3%, respectively) and precision (intraday and interday precision errors were 5.3% and 12.8%, respectively), is shown and discussed. Copyright © 2009 John Wiley & Sons, Ltd.