Premium
Discrimination of cyclic peptide diastereomers by electrospray ionization tandem mass spectrometry
Author(s) -
Zheng Bo,
Liu Yiquan,
Li Huihui,
Ye Yunhua,
Gao Xingming,
Yuan Gu
Publication year - 2009
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1635
Subject(s) - chemistry , diastereomer , protein mass spectrometry , electrospray ionization , tandem mass spectrometry , mass spectrometry , chromatography , selected reaction monitoring , cyclic peptide , extractive electrospray ionization , electrospray , peptide , organic chemistry , biochemistry
In this research, the characteristic ions' abundance ratio between two isomers A and B in MS/MS mass spectra was defined as a parameter for discriminating diastereomers. Through this ratio, the discrimination of four pairs of cyclic peptide (CP) diastereomers was successfully achieved. Furthermore, in the analysis of diastereomers' mixtures, both calibration curve and calculational methods were substantiated to have high precision and accuracy. The average absolute errors of the two methods were 2.0 and 2.5% in the 48 measurements of 16 samples, respectively. This research provided a promising approach for the analysis of the CP diastereomers in the fields of asymmetrical synthesis, chiral natural products and structural biology by ESI‐MS/MS. Copyright © 2009 John Wiley & Sons, Ltd.