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A quantitative GC‐MS method for three major polyamines in postmortem brain cortex
Author(s) -
Chen Gary G.,
Turecki Gustavo,
Mamer Orval A.
Publication year - 2009
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1597
Subject(s) - chemistry , derivatization , putrescine , spermine , spermidine , chromatography , o phthalaldehyde , quantitative analysis (chemistry) , polyamine , chemical ionization , mass spectrometry , biochemistry , ion , ionization , organic chemistry , enzyme
A quantitative method for putrescine (PUT), spermidine (SPD) and spermine (SPM) in homogenized postmortem human brain tissue is described that employs a novel, simple and rapid extractive derivatization with ethylchloroformate and trifluoroacetylation. These amines are metabolites of ornithine and are metabolically interconvertible in mammals. The method was developed to support an ongoing epidemiological study correlating these amines with the frequency of suicide. The isolation methodology is robust and requires less work and time than many previous methods. Analysis is by conventional electron ionization GC‐MS with selected ion monitoring using a stable isotope‐labeled analog for PUT and a chemical analog for SPD and SPM as internal standards. The time required for chromatographic analysis, about 20 min, is determined by the wide range of the relative volatilities of the derivatized polyamines. The method allows the quantitation of PUT down to 10 ng/g and SPD and SPM down to 100 and 1000 ng/g, respectively of wet tissue. Copyright © 2009 John Wiley & Sons, Ltd.