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Determination of polychlorodibenzo‐ p ‐dioxins and polychlorodibenzofurans by gas chromatography/tandem mass spectrometry and gas chromatography/triple mass spectrometry in a quadrupole ion trap
Author(s) -
Helen C.,
Lemasle M.,
Laplanche A.,
Genin E.
Publication year - 2001
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.156
Subject(s) - chemistry , mass spectrometry , tandem mass spectrometry , ion trap , quadrupole ion trap , dissociation (chemistry) , ion , analytical chemistry (journal) , collision induced dissociation , chromatography , triple quadrupole mass spectrometer , gas chromatography , selected reaction monitoring , organic chemistry
The determination of tetra‐ to octachlorodibenzo‐ p ‐dioxins and tetra‐ to octachlorodibenzofurans (PCCD/Fs) by high‐resolution gas chromatography/tandem mass spectrometry (HRGC/MS/MS) and high‐resolution gas chromatography/triple mass spectrometry (HRGC/MS 3 ) in a quadrupole ion trap, equipped with an external ion source, is presented. MS/MS involves a typical four‐step process, namely ionization, parent ion isolation, collision‐induced dissociation (CID) and mass analysis of the daughter ions. For the MS 3 experiment, the MS/MS scan function is used with the addition of selected daughter ion isolation, their CID and the mass analysis of second‐generation product ions called ‘grand‐daughter ions.’ For both methods, the energies necessary for the CID of the 17 PCDD/Fs were determined and optimized using multiple scan functions with different CID amplitudes. The CID efficiency, defined as the signal ratio of fragment ions detected from the major dissociation channels to molecular ions isolated, was 1.15–2.40 V for parent ion dissociation (MS/MS) and 1.05–1.50 V for daughter ion dissociation (MS 3 ) and for all the chloro congeners. The same sensitivity (1 pg µl −1 ) can be reached with both the MS/MS and MS 3 methods and linear responses were obtained between 1 and 100 pg µl −1 injected. Copyright © 2001 John Wiley & Sons, Ltd.