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Separation and identification of purine nucleosides in the urine of patients with malignant cancer by reverse phase liquid chromatography/electrospray tandem mass spectrometry
Author(s) -
Li HuaYu,
Wang ShaoMin,
Liu HongMin,
Bu ShanShan,
Li Juan,
Han Dong,
Zhang Mingzhi,
Wu GuangYin
Publication year - 2009
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1539
Subject(s) - chemistry , inosine , chromatography , purine , mass spectrometry , high performance liquid chromatography , urine , tandem mass spectrometry , guanosine , purine metabolism , liquid chromatography–mass spectrometry , epitestosterone , ribonucleoside , nucleoside , adenosine , stereochemistry , biochemistry , rna , hormone , androgen , enzyme , gene
Urinary‐modified nucleosides have a potential role as cancer biomarkers for a number of malignant diseases. High performance liquid chromatography (HPLC) was combined with full‐scan mass spectrometry, MS/MS analysis and accurate mass measurements in order to identify purine nucleosides purified from urine. Potential purine nucleosides were assessed by their evident UV absorbance in the HPLC chromatogram and then further examined by the mass spectrometric techniques. In this manner, numerous modified purine nucleosides were identified in the urine samples from cancer patients including xanthine, adenosine, N 1‐methyladenosine, 5′‐deoxy‐5′‐methylthioadenosine, 2‐methyladenosine, N 6‐threonylcarbamoyladenosine, inosine, N 1‐methylinosine, guanosine, N 1‐methylguanosine, N 7‐methylguanine, N 2‐methylguanosine, N 2, N 2‐dimethyguanosine, N 2, N 2, N 7‐trimethylguanosine. Furthermore, a number of novel purine nucleosides were tentatively identified via critical interpretation of the combined mass spectrometric data including N 3‐methyladenosine, N 7‐methyladenine, 5′‐dehydro‐2′‐deoxyinosine, N 3‐methylguanine, O 6‐methylguanosine, N 1, N 2, N 7‐trimethylguanosine, N 1‐methyl‐ N 2‐ethylguanosine and N 7‐methyl‐ N 1‐ethylguanosine. Copyright © 2009 John Wiley & Sons, Ltd.