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Study on the redox reactions for organic dyes and S ‐nitrosylated peptide in electrospray droplet impact
Author(s) -
Asakawa Daiki,
Hiraoka Kenzo
Publication year - 2009
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1521
Subject(s) - chemistry , electrospray ionization , mass spectrometry , methylene blue , desorption , ionization , redox , polyatomic ion , protonation , ion , analyte , electrospray , photochemistry , chromatography , analytical chemistry (journal) , inorganic chemistry , organic chemistry , catalysis , photocatalysis , adsorption
Reduction of analytes in ionization processes often obscures the determination of molecular structure. The reduction of analytes is found to take place in various desorption/ionization methods such as fast atom bombardment (FAB), secondary ion mass spectrometry (SIMS), matrix‐assisted laser desorption/ionization (MALDI) and desorption ionization on porous silicon (DIOS). To examine the extent of the reduction reactions taking place in electrospray droplet impact (EDI) processes, reduction‐sensitive dyes and S ‐nitrosylated peptide were analyzed by EDI. No reduction was observed for methylene blue. While methyl red has a lower reduction potential than methylene blue, the reduction product ions were detected. For S ‐nitrosylated peptide, protonated molecule ion [M + H] + and NO‐eliminated molecular ion [M − NO + H] +• were observed but reduction reactions are largely suppressed in EDI compared with that in MALDI. As such, the analytes examined suffer from little reduction reactions in EDI. Copyright © 2008 John Wiley & Sons, Ltd.