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Mass spectrometric studies of the path of carbon in photosynthesis: positional isotopic analysis of 13 C‐labelled C 4 to C 7 sugar phosphates
Author(s) -
MacLeod John K.,
Flanigan Ian L.,
Williams John F.,
Collins J. Grant
Publication year - 2001
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.147
Subject(s) - chemistry , trimethylsilyl , mass spectrum , fragmentation (computing) , electron ionization , sugar phosphates , carbon fibers , photosynthesis , isotopes of carbon , sugar , mass spectrometry , gas chromatography , polyatomic ion , ion , ionization , stereochemistry , organic chemistry , chromatography , total organic carbon , phosphate , biochemistry , materials science , composite number , computer science , composite material , operating system
The (EIMS) electron ionization mass spectrometric fragmentation patterns of the methoxime‐ and ethoxime‐trimethylsilyl (TMS) derivatives of C 4 to C 7 sugars involved as phosphates in the Calvin pathway of photosynthesis in plants were analysed by gas chromatography/EIMS using specifically labelled 13 C analogs. In general, most but not all of the major ions in the mass spectra arise from single carbon–carbon bond cleavages of the straight‐chain derivatives. The results confirm that GC/MS of the alkoxime‐TMS derivatives is a viable method for measuring 13 C incorporations at individual carbon atoms in each of the sugar phosphates during photosynthetic experiments with 13 CO 2 . Copyright © 2001 John Wiley & Sons, Ltd.
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