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Introduction of HPLC/orbitrap mass spectrometry as screening method for doping control
Author(s) -
Virus E. D.,
Sobolevsky T. G.,
Rodchenkov G. M.
Publication year - 2008
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1447
Subject(s) - chemistry , orbitrap , chromatography , repeatability , detection limit , analyte , mass spectrometry , atmospheric pressure chemical ionization , high performance liquid chromatography , analytical chemistry (journal) , chemical ionization , ionization , ion , organic chemistry
A new doping control screening method has been developed, for the analysis of doping agents in human urine, using HPLC/orbitrap with in‐source collision‐induced dissociation and atmospheric pressure chemical ionization. The developed method allows the detection of 29 compounds, including agents with antiestrogenic activity, β 2 agonists, exogenous anabolic steroids, and other anabolic agents. The mass accuracy of this method is better at 2 ppm using an external reference. The detection limit for all compounds tested was better than 100 pg/ml. The recoveries of most analytes were above 70%. The measured median repeatability values for doping agents included in the method at concentrations of 1 and 10 ng/ml were 21 and 17%, respectively. The relative standard deviation (RSD) of the intraday precision ( n = 6) ranged from RSD = 16–22%, whereas the interday precision ( n = 18), ranged from RSD = 17–26%, depending on the solute concentration investigated. Copyright © 2008 John Wiley & Sons, Ltd.