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The application of carbon isotope ratio mass spectrometry to doping control
Author(s) -
Cawley Adam T.,
Flenker Ulrich
Publication year - 2008
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1437
Subject(s) - chemistry , isotope ratio mass spectrometry , mass spectrometry , isotope , isotopes of carbon , analytical chemistry (journal) , chromatography , doping , combustion , gas chromatography , carbon fibers , organic chemistry , total organic carbon , physics , optoelectronics , quantum mechanics , composite number , materials science , composite material
The administration of synthetic steroid copies is one of the most important issues facing sports. Doping control laboratories accredited by the World Anti‐Doping Agency (WADA) require methods of analysis that allow endogenous steroids to be distinguished from their synthetic analogs in urine. The ability to measure isotope distribution at natural abundance with high accuracy and precision has increased the application of Gas Chromatography–Combustion–Isotope Ratio Mass Spectrometry (GC–C–IRMS) to doping control in recent years. GC–C–IRMS is capable of measuring the carbon isotope ratio (δ 13 C) of urinary steroids and confirm their synthetic origin based on the abnormal 13 C content. This tutorial describes some of the complexities encountered by obtaining valid δ 13 C measurements from GC–C–IRMS and the need for careful interpretation of all relevant information concerning an individual's metabolism in order to make an informed decision with respect to a doping violation. Copyright © 2008 John Wiley & Sons, Ltd.