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A high‐throughput multicomponent screening method for diuretics, masking agents, central nervous system (CNS) stimulants and opiates in human urine by UPLC–MS/MS
Author(s) -
Thörngren JohnOlof,
Östervall Fredrik,
Garle Mats
Publication year - 2008
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1436
Subject(s) - chemistry , chromatography , tandem mass spectrometry , mass spectrometry , electrospray ionization , pipette , electrospray , high performance liquid chromatography , sample preparation , central nervous system , analytical chemistry (journal) , neuroscience , biology
A simple and rapid multicomponent screening method of 130 substances for direct injections of urine samples has been developed. The fully automated method based on ultra‐performance liquid chromatography (UPLC) and tandem mass spectrometry (MS/MS) is used for three different classes of doping agents: diuretics, central nervous system stimulants (CNS stimulants) and opiates. The samples are diluted with buffer containing internal standards (IS) by a pipetting robot system into 96‐well plates. Samples are injected on a reversed phase sub 2‐µm particle column connected to a fast polarity switching and rapid scanning tandem mass spectrometer with an electrospray interface. The software used to evaluate the results produced reports containing a small‐sized window for each component and a data table list with flags to indicate any adverse analytical findings in the sample. The report can also be processed automatically using an application software, which interpret the data and indicate if there is a suspicious sample. One 96‐well plate can be analyzed within 16 h. Copyright © 2008 John Wiley & Sons, Ltd.