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Fragmentation patterns of newly isolated cassane butenolide diterpenes and differentiation of stereoisomer by tandem mass spectrometry
Author(s) -
Kanojiya Sanjeev,
Yadav Prem P.
Publication year - 2008
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1422
Subject(s) - chemistry , fragmentation (computing) , tandem mass spectrometry , mass spectrometry , electrospray ionization , collision induced dissociation , stereochemistry , electrospray , mass spectrum , chromatography , analytical chemistry (journal) , computer science , operating system
Different stereoisomers of active molecules often cause different physiological responses and hence pose a challenge for their identification. This study involves perceptive fragmentation behavior of newly isolated cassane butenolides, caesalpinolide A [1] and caesalpinolide B [2] (epimeric at the hemiketal position) by tandem MS. The electrospray ionization‐mass spectrometry (ESI‐MS)/collision‐induced dissociation (CID; ESI‐MS 2 and ESI‐IT‐MS n ) were investigated. The effect of orientations of hemiketal hydroxyl at C‐12 was clearly observed in the mass spectrum. Tandem mass spectra of 1, 1 A or 2, 2 A show stereospecific fragmentation resulting in significant abundance dissimilarity of [MH − H 2 O] + as well as differences in fragmentation pathway. Both of these pathways seem to be influenced by the stereochemistry of the molecule. The differentiation can be clearly visualized from the [M + H − H 2 O] + /[M + H] + ratio of the two isomers where β‐isomer 2 was found to be five times higher than that of α‐isomer 1 in full scan liquid chromatography‐electrospray ionization mass spectrometry(LC‐ESI‐MS). In high‐energy CID, the mass fingerprint of 1, 2, 1 A , and 2 A was found to be different from one anothers. Copyright © 2008 John Wiley & Sons, Ltd.