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Evaluation of a new matrix‐free laser desorption/ionization method through statistic studies: comparison of the DIAMS (desorption/ionization on self‐assembled monolayer surface) method with the MALDI and TGFA‐LDI techniques
Author(s) -
Bounichou Matthieu,
Sanguinet Lionel,
Elouarzaki Kamal,
Alévêque Olivier,
Dias Marylène,
Levillain Eric,
Rondeau David
Publication year - 2008
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1414
Subject(s) - chemistry , matrix assisted laser desorption electrospray ionization , desorption , soft laser desorption , atmospheric pressure laser ionization , matrix assisted laser desorption/ionization , analytical chemistry (journal) , mass spectrometry , surface enhanced laser desorption/ionization , ionization , monolayer , detection limit , ambient ionization , matrix (chemical analysis) , repeatability , chromatography , chemical ionization , ion , adsorption , photoionization , organic chemistry , biochemistry
This work demonstrates that the desorption/ionization on self‐assembled monolayer surface (DIAMS) mass spectrometry, a recent matrix‐free laser desorption/ionization (LDI) method based on an organic target plate, is as statistically repeatable and reproducible as matrix assisted laser desorption ionization (MALDI) and thin gold film‐assisted laser desorption/ionization (TGFA‐LDI) mass spectrometries. On lipophilic DIAMS of target plates with a mixture of glycerides, repeatability/reproducibility has been estimated at 15 and 30% and the relative detection limit has been evaluated at 0.3 and 3 pmol, with and without NaI respectively. Salicylic acid and its d 6 ‐isomer analysis confirm the applicability of the DIAMS method in the detection of compounds of low molecular weight. Copyright © 2008 John Wiley & Sons, Ltd.