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Mass spectrometric analysis of the marine lipophilic biotoxins pectenotoxin‐2 and okadaic acid by four different types of mass spectrometers
Author(s) -
Gerssen Arjen,
Mulder Patrick,
van Rhijn Hans,
de Boer Jacob
Publication year - 2008
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1407
Subject(s) - chemistry , mass spectrometry , electrospray , fragmentation (computing) , chromatography , ion trap , analytical chemistry (journal) , ion , selected reaction monitoring , triple quadrupole mass spectrometer , okadaic acid , mass spectrum , quadrupole time of flight , electrospray ionization , tandem mass spectrometry , organic chemistry , computer science , phosphatase , enzyme , operating system
The performances of four different mass spectrometers [triple‐quadrupole (TQ), time‐of‐flight (ToF), quadrupole ToF (Q‐ToF) and ion trap (IT)] for the detection of the marine lipophilic toxins pectenotoxin‐2 (PTX2) and okadaic acid (OA) were investigated. The spectral data obtained with the different mass spectrometric analyzers were used to propose fragmentation schemes for PTX2 in the positive electrospray mode and for OA in the negative electrospray mode. TQ data were used to obtain product ions, while ToF and Q‐ToF‐MS produced accurate mass data of the precursor ion and product ions, respectively. IT data provided a better understanding of the fragmentation pathways using MS n experiments. With respect to analytical performance, all four mass analyzers showed a good linearity ( R 2 > 0.97) and repeatability (CV < 20%). Detection limits (LoDs) (S/N = 3) were the lowest on triple‐quad MS: 12.2 and 2.9 pg on‐column for PTX2 and OA, respectively. Copyright © 2008 John Wiley & Sons, Ltd.