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MALDI mass spectrometric determination of dendritic iron chelation stoichiometries and conditional affinity constants
Author(s) -
Kong Xiaole,
Neubert Hendrik,
Zhou Tao,
Liu ZuDong,
Hider Robert C.
Publication year - 2008
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1357
Subject(s) - chemistry , chelation , stoichiometry , stability constants of complexes , cooperativity , mass spectrometry , inorganic chemistry , chromatography , aqueous solution , biochemistry
Abstract The iron chelation stoichiometries of a dendritic iron(III) chelator with N 1 , N 3 , N 5 ‐trimethylbenzene‐1,3,5‐tricarboxamide at its core, and containing 3 identical hexadentate tris‐hydroxypyridinone branches D was studied by MALDI mass spectrometry. At pH 7.2, the speciation of the system included FeD, Fe 2 D and Fe 3 D species with the respective conditional stability constants of 26.74, 26.03 and 25.36. The differences in the stepwise affinity constants arise from the statistical distribution of iron(III), and there was no evidence for cooperativity between the iron‐binding sites. Copyright © 2008 John Wiley & Sons, Ltd.