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Self‐assembled‐monolayer‐modified silicon substrate to enhance the sensitivity of peptide detection for AP‐MALDI mass spectrometry
Author(s) -
Hsieh Shuchen,
Ku HsinYi,
Ke YaoTang,
Wu HuiFen
Publication year - 2007
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1261
Subject(s) - chemistry , substrate (aquarium) , mass spectrometry , monolayer , chromatography , silicon , analytical chemistry (journal) , self assembled monolayer , gramicidin , desorption , gramicidin s , detection limit , matrix assisted laser desorption/ionization , adsorption , organic chemistry , biochemistry , oceanography , membrane , geology
Abstract A self‐assembled‐monolayer‐modified silicon substrate was successfully used to enhance the sensitivity of peptide detection for atmospheric pressure‐matrix‐assisted laser desorption/ionization mass spectrometry (AP‐MALDI/MS). The effect of surface modification of silicon wafer samples with NH 2 and OH functional groups was investigated. In addition, solvent effects for the preparation of modified NH 2 ‐functionalized surfaces were examined. The sensitivities for the two peptides were significantly improved, increasing between 12 and 160 times, for bradykinin and gramicidin, respectively, on an NH 2 ‐modified silicon surface prepared in toluene, over that on a conventional gold substrate. The limits of detection (LODs) for bradykinin and gramicidin using the conventional gold substrate in AP‐MALDI/MS experiments were > 0.011 µM and 110 µM, respectively. Using our SAM approach, the LODs for bradykinin and gramicidin in AP‐MALDI/MS can be improved to 0.93 nM and 0.33 µM, respectively. This SAM approach for AP‐MALDI/MS is simple and sensitive, and can be used for high‐throughput analysis. Copyright © 2007 John Wiley & Sons, Ltd.