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Profiling and imaging of tissues by imaging ion mobility‐mass spectrometry
Author(s) -
McLean John A.,
Ridenour Whitney B.,
Caprioli Richard M.
Publication year - 2007
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1254
Subject(s) - chemistry , ion mobility spectrometry , mass spectrometry , mass spectrometry imaging , ion , analyte , profiling (computer programming) , isobaric process , maldi imaging , analytical chemistry (journal) , chromatography , matrix assisted laser desorption/ionization , adsorption , desorption , computer science , thermodynamics , operating system , physics , organic chemistry
Molecular profiling and imaging mass spectrometry (IMS) of tissues can often result in complex spectra that are difficult to interpret without additional information about specific signals. This report describes increasing data dimensionality in IMS by combining two‐dimensional separations at each spatial location on the basis of imaging ion mobility‐mass spectrometry (IM‐MS). Analyte ions are separated on the basis of both ion‐neutral collision cross section and m / z , which provides rapid separation of isobaric, but structurally distinct ions. The advantages of imaging using ion mobility prior to MS analysis are demonstrated for profiling of human glioma and selective lipid imaging from rat brain. Copyright © 2007 John Wiley & Sons, Ltd.