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Evaluation of relative DNA binding affinities of anthrapyrazoles by electrospray ionization mass spectrometry
Author(s) -
Smith Suncerae I.,
Guziec Lynn J.,
Guziec Frank S.,
Hasinoff Brian B.,
Brodbelt Jennifer S.
Publication year - 2007
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1205
Subject(s) - chemistry , electrospray ionization , mass spectrometry , dna , affinities , dissociation (chemistry) , electrospray , binding affinities , ligand (biochemistry) , chromatography , stereochemistry , organic chemistry , biochemistry , receptor
Binding interactions of a new series of anthrapyrazoles (APs) with DNA were evaluated by electrospray ionization mass spectrometry (ESI‐MS). Relative binding affinities were estimated from the ESI‐MS data based on the fraction of bound DNA for DNA/anthrapyrazole mixtures, and they show a correlation to the shift in melting point of the DNA measured from a previous study. Minimal sequence specificity was observed for the series of anthrapyrazoles. Upon collisionally activated dissociation of the duplex/anthrapyrazole complexes, typically ejection of the ligand was the dominant pathway for most of the complexes. However, for complexes containing AP2 or mitoxantrone, strand separation with the ligand remaining on one of the single strands was observed, indicative of a different binding mode or stronger binding. Copyright © 2007 John Wiley & Sons, Ltd.