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A micromethod for the measurement of deuterium bound to carbon‐6 of glucose to quantify gluconeogenesis in vivo
Author(s) -
Kalhan Satish C.,
Trivedi Roopa,
Singh Sheila,
Chandramouli Visvanathan,
Schumann William C.,
Landau Bernard R.
Publication year - 1995
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1190301111
Subject(s) - chemistry , formaldehyde , deuterium , in vivo , hexamethylenetetramine , yield (engineering) , mass spectrometry , chromatography , organic chemistry , physics , materials science , microbiology and biotechnology , quantum mechanics , metallurgy , biology
A method to measure the enrichment of deuterium in the hydrogens bound to C‐6 of glucose has been refined and taken to the micro‐scale. Glucose (≤0.2 mg) in small quantities of plasma samples is oxidized with sodium periodate. The formaldehyde formed contains the two hydrogens bound to C‐6 of glucose. The formaldehyde is condensed with ammonia to form hexamethylenetetramine (HMT), which can be directly determined by gas chromatography/mass spectrometry. All of the 12 hydrogens of HMT are from the formaldehyde and therefore deuterium enrichment as low as 0.1% on hydrogens bound to C‐6 of glucose can be measured with a coefficient of variation of less than 3%. The method can be used to quantify gluconeogenesis in vivo in humans by administering [ 2 H 2 ]O at an enrichment in total body water of only 0.5%, resulting in reproducibly measurable labeling of the hydrogens bound to C‐6 of glucose. The method is presented because of its potential value for the measurement of gluconeogenesis in humans in vivo at safe and acceptable doses of [ 2 H 2 ]O.