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Liquid chromatography/fast atom bombardment and electrospray ionization mass spectrometry of gibberellin A 3 1,2‐ trans ‐glycosyl esters
Author(s) -
Schneider Gernot,
Schmidt Jürgen,
Moritz Thomas
Publication year - 1995
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1190301015
Subject(s) - chemistry , fast atom bombardment , glycosyl , electrospray ionization , mass spectrometry , electrospray , fragmentation (computing) , chromatography , tandem mass spectrometry , glycoside , organic chemistry , computer science , operating system
The glucosyl ester (2b), the galactosyl ester (3b), the xylosyl ester (4b) and the arabinosyl ester (5b) of gibberellin A 3 (GA 3 ) as well as the glucosyl ester (7b) and the xylosyl ester (8b) of isoGA 3 were synthesized and their structures confirmed especially by 1 H nuclear magnetic resonance. In order to identify such conjugates in nature, the glycosyl esters 2b, 3b, 4b, 5b, 7b and 8b were subjected to mass spectrometry analysis using fast atom bombardment and electrospray ionization techniques. The spectra obtained from both negative and positive ions were compared and interpreted on the basis of tandem mass spectrometry experiments. The differences between the fragmentation of GA 3 glycosyl ester and that of isoGA 3 glucosyl ester are discussed. Diagnostic ions were evaluated, which should be useful for the identification of these conjugates by liquid chromatography/mass spectrometry.