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Matrix‐assisted laser desorption/ionization mass spectrometry (MALDI‐MS) of membrane proteins and non‐covalent complexes
Author(s) -
Rosinke Burkhard,
Strupat Kerstin,
Hillenkamp Franz,
Rosenbusch Jürg,
Dencher Norbert,
Krüger Ulrike,
Galla HansJoachim
Publication year - 1995
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1190301012
Subject(s) - chemistry , porin , bacteriorhodopsin , mass spectrometry , matrix assisted laser desorption/ionization , desorption , mass spectrum , chromatography , halobacterium salinarum , matrix assisted laser desorption electrospray ionization , analyte , analytical chemistry (journal) , membrane , escherichia coli , bacterial outer membrane , organic chemistry , biochemistry , adsorption , gene
Matrix‐assisted laser desorption/ionization (MALDI) mass spectra and methods to improve their quality are reported for three hydrophobic, membrane‐bound proteins: porin from Escherichia coli , bacteriorhodopsin from Halobacterium salinarium and cholesterolesterase from Pseudomonas fluorescens. Several commonly used UV and IR matrices have been tested. In addition, the susceptibility of MALDI mass spectrometry to various neutral and ionic detergents, known usually to degrade the quality of MALDI mass spectra, has been tested systematically. For porin, consisting of three identical non‐covalently bound subunits, a new sample preparation is reported, resulting in the desorption of the intact quaternary protein structure. This leads to a better understanding of the way a given analyte is embedded into the host matrix crystals.