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Stable isotope labelling and quantitative determination of enalaprilate in human plasma by GC–NICI–MS
Author(s) -
Leis H. J.,
Gleispach H.,
Windischhofer W.
Publication year - 1995
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1190301010
Subject(s) - chemistry , metabolite , chromatography , detection limit , chemical ionization , mass spectrometry , gas chromatography–mass spectrometry , extraction (chemistry) , gas chromatography , solid phase extraction , active metabolite , quantitative analysis (chemistry) , ionization , ion , organic chemistry , biochemistry
A method for the quantitative determination of enalaprilate, the active metabolite of the angiotensin‐converting enzyme inhibitor enalapril, is presented. The asay is based on gas chromatography–negative ion chemical ionization–mass spectrometry. The preparation of 18 O 4 ‐labelled enalaprilate for use as an internal standard is described. The method involves solid phase extraction and derivation to the pentafluorobenzyl diester–trifluoroacetamide derivatives. The detection limit was found to be 0.5 pg, and a lower limit of quantitation was reached down to 40 pg ml −1 plasma. The method was applied to pharmacokinetics of the metabolite in humans.