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Determination of both linkage position and anomeric configuration in underivatized glucopyranosyl disaccharides by electrospray mass spectrometry
Author(s) -
Mulroney Brad,
Traeger John C.,
Stone Bruce A.
Publication year - 1995
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1190300911
Subject(s) - chemistry , anomer , glycosidic bond , derivatization , disaccharide , mass spectrometry , electrospray , tandem mass spectrometry , electrospray mass spectrometry , chromatography , electrospray ionization , linkage (software) , analytical chemistry (journal) , glycan , stereochemistry , organic chemistry , biochemistry , enzyme , gene , glycoprotein
Negative‐ion electrospray mass spectrometry was used to determine directly both glycosidic linkage position and anomeric configuration in the complete glucopyranosyl disaccharide series without the need for derivatization or tandem mass analysis. A series of empirical criteria were developed which first differentiate between the linkage positions and then the anomeric configurations using selected diagnostic peaks and their relative intensities. The use of standard mass spectral library searching methods for structural analysis was also investigated.