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Determination of various steroids in normal adrenal glands and adrenocortical tumours by liquid chromatography/atmospheric pressure ionization mass spectrometry
Author(s) -
Komatsu Funito,
Morioka Masaaki,
Fujita Yukitoshi,
Sugahara Kazunori,
Kodama Hiroyuki
Publication year - 1995
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1190300507
Subject(s) - chemistry , chromatography , corticosterone , aldosterone , atmospheric pressure chemical ionization , androstenedione , mass spectrometry , endocrinology , mineralocorticoid , medicine , chemical ionization , hydroxyprogesterone , adrenal gland , ion , steroid , ionization , hormone , androgen , biochemistry , organic chemistry
Seven steroids in normal adrenal gland and those of patients with aldosteronoma or Cushing's adenoma were measured by liquid chromatography/mass spectrometry with an atmospheric pressure ionization interface system. Using this system, quasi‐molecular ions [M + H] + of standard cortisol, corticosterone, 17α‐hydroxyprogesterone, 11β‐hydroxyandrostenedione and Δ 4 ‐androstenedione and [(M + H) − H 2 O] + ions of aldosterone and dehydroe‐piandrosterone were observed as their base peaks, and quasi‐molecular ions [M + H] + and [(M + H) − H 2 O] + ions of seven steroids were clearly observed in chromatograms of biological samples. The ratio of glucocorticoid (cortisol) to mineralocorticoid (corticosterone) content was measured, and found to be significantly lower for aldosteronomas and higher for Cushing's adenomas than for normal adrenals. These findings indicate that the method had suffficient sensitivity for the identification and simultaneous measurement of a variety of adrenal gland steroids.