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Thin‐layer chromatography/laser desorption of peptides followed by multiphoton ionization time‐of‐flight mass spectrometry
Author(s) -
Krutchinsky A. N.,
Dolgin A. I.,
Utsal O. G.,
Khodorkovski A. M.
Publication year - 1995
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1190300221
Subject(s) - chemistry , mass spectrometry , ionization , chromatography , analytical chemistry (journal) , thin layer chromatography , mass spectrum , time of flight mass spectrometry , laser , surface enhanced laser desorption/ionization , desorption , cellulose , direct electron ionization liquid chromatography–mass spectrometry interface , sample preparation in mass spectrometry , chemical ionization , electrospray ionization , adsorption , ion , optics , organic chemistry , physics
The location of the separated compounds of GlnTrp/GlyTyr and pentagastrin/gramicidin D peptide model mixtures on silica gel and cellulose thin‐layer chromatography plates has been examined by laser desorption multiphoton ionization time‐of‐flight mass spectrometry. The multiphoton ionization mass spectra of neutrals desorbed by sequential scanning of the thin‐layer chromatography plates versus the infrared laser spot allowed imaging of the distribution profile of the compounds. The method appeared to be promising for the analysis of thin‐layer chromatograms.