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Cation‐exchange resins for removal of alkali metal cations from oligonucleotide samples for fast atom bombardment mass spectrometry
Author(s) -
Vollmer David L.,
Gross Michael L.
Publication year - 1995
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1190300117
Subject(s) - chemistry , fast atom bombardment , alkali metal , salt (chemistry) , ion exchange resin , mass spectrometry , ion exchange , matrix (chemical analysis) , ion , nucleotide , metal , nuclear chemistry , inorganic chemistry , chromatography , analytical chemistry (journal) , organic chemistry , biochemistry , gene
Cation‐exchange resins are useful matrix additives for the positive and negative ion FAB‐MS determination of nucleotides. Several factors including the amount and type of matrix, the amount and type of the resin, the molecular weight of the nucleotide, and the salt content of the nucleotide sample are investigated. For positive ions, the most abundant [M + H] + ions are obtained with 1 μL of DTT/DTE with 75–100 resin beads in the H + form is used. For negative ions, the most abundant [M − H] − ions are produced from 1 μL TEA and 75–100 resin beads in the NH 4 + form. In several cases, the use of cation‐exchange resins give superiors results compared to those obtained with other matrix additives.