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Tandem mass specatrometry of carbonic anhydrase (29 kDa)
Author(s) -
O'Connor Peter B.,
Speir J. Paul,
Senko Michael W.,
Little Daniel P.,
McLafferty Fred W.
Publication year - 1995
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1190300114
Subject(s) - chemistry , carbonic anhydrase , tandem , tandem mass spectrometry , carbonic anhydrase ii , chromatography , biochemistry , enzyme , mass spectrometry , materials science , composite material
Using electrospray ionization with a Fourier‐transform mass spectrometer, MS/MS of molecular ions and seven fragment ions of carbonic anhydrase (259 amino acids) yields 74 identifiable fragment ions, including 23 amino acid spacings. Such sensitive and specific direct characterization of proteins appears to be complementary to conventional techniques including LC/MS/MS sequencing of oligopeptides from protein degradation.