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Differential fragmentation patterns of pectin oligogalacturonides observed by nanoelectrospray quadrupole ion‐trap mass spectrometry using automated spectra interpretation
Author(s) -
Mutenda Kudzai E.,
Matthiesen Rune,
Roepstorff Peter
Publication year - 2007
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1169
Subject(s) - chemistry , fragmentation (computing) , glycosidic bond , mass spectrometry , ion , mass spectrum , tandem mass spectrometry , stereochemistry , chromatography , biochemistry , organic chemistry , enzyme , computer science , operating system
Oligogalacturonides of different degrees of polymerization (DP) and methyl esterification (DE) were structurally analyzed by nanoESI quadrupole ion‐trap mass spectrometry. The fragmentation patterns of the oligogalacturonides were compared using the program ‘Virtual Expert Mass Spectrometrist’ (VEMS) for structural annotation. In the analyzed oligogalacturonides of lower DP, the generation of C/Y ions, i.e. ions retaining the glycosidic oxygen, was higher than that of B/Z ions. In general, with oligogalacturonides of higher DP, the B/Z ions were generated more abundantly. Oligogalacturonides with free carboxylic acid groups underwent higher water loss compared to fully methyl‐esterified oligogalacturonides under the same fragmentation conditions. Cross‐ring cleavage, in which fragmentation occurs across the ring system of the galacturonate residue and signified by unique mass losses, was observed to be higher in fully methyl‐esterified oligogalacturonides than in non‐methyl‐esterified ones. This study demonstrates the different fragmentation patterns of oligogalacturonides as influenced by the presence or absence of methyl ester groups. For a detailed analysis of unknown oligogalacturonides, cross‐ring fragmentation gives more structural information than glycosidic bond cleavage. One implication of this is that more structural information is obtained when analyzing methyl‐esterified oligogalacturonides than non‐methyl‐esterified ones in an ion‐trap instrument. This is of particular importance in pectin chemistry, where mass spectrometry has become the technique of choice for structural determination. Although this study was not designed to explain the mechanisms of oligogalacturonide fragmentation, possible explanations for why non‐methyl‐esterified oligogalacturonides undergo more water loss than methyl‐esterified ones will be postulated. In addition, the VEMS program was extended to automatically interpret and assign the fragment ions peaks generated in this study. Copyright © 2007 John Wiley & Sons, Ltd.