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Open‐tubular capillary electrochromatography‐mass spectrometry with sheathless nanoflow electrospray ionization for analysis of amino acids and peptides
Author(s) -
Chen Zilin,
Boggess Bill,
Chang HsuehChia
Publication year - 2007
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1158
Subject(s) - chemistry , capillary electrochromatography , electrospray , chromatography , electrospray ionization , mass spectrometry , electrochromatography , capillary electrophoresis–mass spectrometry , capillary action , analyte , analytical chemistry (journal) , capillary electrophoresis , materials science , composite material
A novel, rugged sheathless capillary electrochromatography‐electrospray ionization (CEC‐ESI) device, in which an open‐tubular separation capillary and an electrospray tip are integrated with a Nafion tubing junction, is coupled to mass spectrometry (MS) for the analysis of amino acids and peptides. A stable electrospray was generated at nanoflow rates by applying a positive electrical potential at the Nafion membrane junction. To sustain the stable spray, an electroosmotic flow (EOF) to the spray was supported by coating the fused silica capillary with Lupamin, a high‐molecular‐weight linear positively charged polyvinylamine (PVAm) polymer, which also minimizes analyte adsorption. Electrochromatographic separation of amino acids and peptides was further enhanced by the chromatographic selectivity of Lupamin stationary phase for these molecules. The device was very reliable and reproducible for CEC‐ESI‐MS analyses of amino acids and peptides for over a hundred injections. The separation and detection behaviors of amino acids and peptides under different conditions including pH, concentration, and composition of mobile phases on Lupamin‐coated and uncoated capillaries have been investigated. The relationship between nano electrospray stability and EOF is discussed. Copyright © 2006 John Wiley & Sons, Ltd.