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Comparison of the medium molecular weight venom fractions from five species of common social wasps by MALDI‐TOF spectra profiling
Author(s) -
Turillazzi Stefano,
Bruschini Claudia,
Lambardi Duccio,
Francese Simona,
Spadolini Igino,
Mastrobuoni Guido
Publication year - 2007
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1152
Subject(s) - chemistry , venom , matrix assisted laser desorption/ionization , mass spectrum , chromatography , mass spectrometry , zoology , desorption , biochemistry , organic chemistry , adsorption , biology
The average spectral profiles and the exact mass weight (MW) of biomolecules present in the medium fraction (from 900 to 3000 Da) of the venom of five social wasps (three European and one North American Polistes and the European hornet Vespa crabro ) were determined by matrix assisted laser desorption ionization time of flight (MALDI‐TOF) MS. Data were obtained analyzing the venom of single specimens ( N = 46) and elaborated with the ClinProTools 2.0 (CPT) software to search for differences among the five species examined. Interesting differences in the spectral profiles were found, allowing the discrimination of venoms belonging to the different species, and their possible use as a quality control method in venom immunotherapy (VIT) for allergic patients. Copyright © 2006 John Wiley & Sons, Ltd.