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Direct analysis of alkaloid profiling in plant tissue by using matrix‐assisted laser desorption/ionization mass spectrometry
Author(s) -
Wu Wei,
Liang Zhitao,
Zhao Zhongzhen,
Cai Zongwei
Publication year - 2007
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1138
Subject(s) - chemistry , mass spectrometry , alkaloid , mass spectrometry imaging , chromatography , matrix assisted laser desorption/ionization , desorption , analytical chemistry (journal) , surface enhanced laser desorption/ionization , matrix (chemical analysis) , environmental chemistry , protein mass spectrometry , tandem mass spectrometry , organic chemistry , adsorption
A method for the direct determination of alkaloid profiling in plant tissues by using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOFMS) was developed. The alkaloid profiles of the herbs were obtained without the need for complicated sample preparation. Experimental results demonstrated that the direct MALDI‐TOFMS analysis allowed rapid and reliable characterization of the components in plant tissues. Four commonly used Chinese medicinal herbs were studied, including Aconitum Carmichaeli Debx. (Fuzi in Chinese) and Processed Fuzi, for herb differentiation and explanation of the significant difference in their toxicities. The direct analysis method proved valuable for the preliminary study of plant component profiles. The rapid collection of information from the direct analysis on plant tissues could be valuable for supporting the discovery of new compounds and for the quality control of medicinal herbs. Copyright © 2006 John Wiley & Sons, Ltd.