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The metabolism of norethandrolone in the horse: characterization of 16‐, 20‐ and 21‐oxygenated metabolites by gas chromatography/mass spectrometry
Author(s) -
McKinney Andrew R.,
Ridley Damon D.,
Suann Craig J.
Publication year - 2001
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.111
Subject(s) - chemistry , chromatography , trimethylsilyl , mass spectrometry , urine , metabolite , gas chromatography–mass spectrometry , metabolism , gas chromatography , horse , anabolic steroid , anabolism , biochemistry , organic chemistry , paleontology , biology
After oral administration to a thoroughbred gelding, the anabolic steroid norethandrolone was converted into a complex mixture of oxygenated metabolites. These metabolites were extracted from the urine, deconjugated by methanolysis and converted to their O ‐methyloxime trimethylsilyl derivatives. Gas chromatographic/mass spectrometric analysis indicated the major metabolites to be 19‐norpregnane‐3,16,17‐triols, 19‐norpregnane‐3,17,20‐triols and 3,17‐dihydroxy‐19‐norpregnan‐21‐oic acids. Some minor metabolites were also detected. Copyright © 2001 John Wiley & Sons, Ltd.