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Sensitive inductively coupled plasma mass spectrometry assay for the determination of platinum originating from cisplatin, carboplatin, and oxaliplatin in human plasma ultrafiltrate
Author(s) -
Brouwers E. E. M.,
Tibben M. M.,
Rosing H.,
Hillebrand M. J. X.,
Joerger M.,
Schellens J. H. M.,
Beijnen J. H.
Publication year - 2006
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1087
Subject(s) - oxaliplatin , chemistry , carboplatin , cisplatin , chromatography , platinum , inductively coupled plasma mass spectrometry , bioanalysis , mass spectrometry , pharmacokinetics , pharmacology , cancer , biochemistry , chemotherapy , colorectal cancer , medicine , surgery , catalysis
Abstract We present a highly sensitive, rapid method for the determination of platinum originating from the anticancer agents cisplatin, carboplatin, and oxaliplatin in human plasma ultrafiltrate. The method is based on the quantification of platinum by inductively coupled plasma mass spectrometry and allows quantification of 7.50 ng l −1 platinum in only 150 µl of matrix. Sample pretreatment involves dilution of samples with 1% HNO 3 . Validation fulfilled the most recent FDA guidelines for bioanalytical method validation. Validated ranges of quantification were 7.50 ng l −1 to 1.00 × 10 5 ng l −1 in plasma ultrafiltrate for all three platinum compounds. The assay is now successfully used to support pharmacokinetic studies in cancer patients treated with cisplatin, carboplatin, or oxaliplatin. Copyright © 2006 John Wiley & Sons, Ltd.