Premium
A validated method for the determination of nicotine, cotinine, trans ‐3′‐hydroxycotinine, and norcotinine in human plasma using solid‐phase extraction and liquid chromatography‐atmospheric pressure chemical ionization‐mass spectrometry
Author(s) -
Kim Insook,
Huestis Marilyn A.
Publication year - 2006
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1039
Subject(s) - chemistry , cotinine , chromatography , analyte , atmospheric pressure chemical ionization , solid phase extraction , mass spectrometry , extraction (chemistry) , nicotine , detection limit , analytical chemistry (journal) , chemical ionization , ionization , ion , organic chemistry , neuroscience , biology
A liquid chromatographic‐mass spectrometric method for the simultaneous determination of nicotine, cotinine, trans ‐3′‐hydroxycotinine, and norcotinine in human plasma was developed and validated. Analytes and deuterated internal standards were extracted from human plasma using solid‐phase extraction and analyzed by liquid chromatography/atmospheric pressure chemical ionization‐mass spectrometric detection with selected ion monitoring (SIM). Limits of detection and quantification were 1.0 and 2.5 ng/ml, respectively, for all analytes. Linearity ranged from 2.5 to 500 ng/ml of human plasma using a weighting factor of 1/ x ; correlation coefficients for the calibration curves were > 0.99. Intra‐ and inter‐assay precision and accuracy were < 15.0%. Recoveries were 108.2–110.8% nicotine, 95.8–108.7% cotinine, 90.5–99.5% trans ‐3′‐hydroxycotinine, and 99.5–109.5% norcotinine. The method was also partially validated in bovine serum, owing to the difficulty of obtaining nicotine‐free human plasma for the preparation of calibrators and quality control (QC) samples. This method proved to be robust and accurate for the quantification of nicotine, cotinine, trans ‐3′‐hydroxycotinine, and norcotinine in human plasma collected in clinical studies of acute nicotine effects on brain activity and on the development of neonates of maternal smokers. Copyright © 2006 John Wiley & Sons, Ltd.