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Atmospheric pressure photoionization‐mass spectrometry and atmospheric pressure chemical ionization‐mass spectrometry of neurotransmitters
Author(s) -
Kauppila T. J.,
Nikkola T.,
Ketola R. A.,
Kostiainen R.
Publication year - 2006
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1034
Subject(s) - chemistry , atmospheric pressure chemical ionization , photoionization , mass spectrometry , atmospheric pressure , chemical ionization , chromatography , analytical chemistry (journal) , formic acid , methanol , ion , ionization , organic chemistry , oceanography , geology
A group of five neurotransmitters with different properties was analyzed using atmospheric pressure photoionization (APPI) and atmospheric pressure chemical ionization‐mass spectrometry (APCI‐MS). The sensitivity of the techniques for the analytes was tested in six solvents and in positive and negative ion modes. APPI was found to be superior in sensitivity for all the compounds in both positive and negative ion modes. In positive ion mode, water/methanol/formic acid was found to be the best solvent, whereas in negative ion mode, water/methanol/ammonium hydroxide performed best. Detection limits using APPI were between 2.5–250 fmol, depending on the compound. The sensitivity was best for the neurosteroids dehydroepiandrosterone and β‐estradiol, and acetylcholine (LOD 2.5–10 fmol). Copyright © 2006 John Wiley & Sons, Ltd.