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Identification of metabolites of 4,4′‐methylenedianiline in vascular smooth muscle cells by liquid chromatography‐electrospray tandem mass spectrometry
Author(s) -
Chen Kan,
Dugas Tammy R.,
Cole Richard B.
Publication year - 2006
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.1026
Subject(s) - chemistry , chromatography , tandem mass spectrometry , liquid chromatography–mass spectrometry , electrospray , mass spectrometry , high performance liquid chromatography , electrospray ionization , electrospray mass spectrometry , diacetyl , biochemistry
A capillary liquid chromatography‐electrospray tandem mass spectrometry (LC‐ES‐MS/MS) method was developed for the identification of metabolites of 4,4′‐methylenedianiline (diaminodiphenyl methane, (DAPM)) derived from exposure of vascular smooth muscle cells (VSMC) to this compound. The use of precolumn concentration and column switching techniques prior to reversed‐phase liquid chromatography coupled online to ES‐MS enabled the separation and detection of low‐level DAPM and its metabolites in the exposed cell samples. The employed LC‐ES‐MS method, and further LC‐ES‐MS/MS analysis enabled the structural assignments for two DAPM metabolites from vascular smooth muscle cells: N ‐acetyl methylenedianiline ( N ‐acetyl‐DAPM) and N , N ′‐diacetyl methylenedianiline ( N , N ′‐diacetyl‐DAPM). Copyright © 2006 John Wiley & Sons, Ltd.