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Collision‐induced dissociation of ring‐opened cyclic depsipeptides with a guanidino group by electrospray ionization/ion trap mass spectrometry
Author(s) -
Kuroda Jun,
Fukai Toshio,
Nomura Taro
Publication year - 2001
Publication title -
journal of mass spectrometry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.475
H-Index - 121
eISSN - 1096-9888
pISSN - 1076-5174
DOI - 10.1002/jms.101
Subject(s) - chemistry , depsipeptide , electrospray ionization , collision induced dissociation , mass spectrometry , ion trap , dissociation (chemistry) , ion , electrospray , protonation , mass spectrum , molecule , stereochemistry , tandem mass spectrometry , chromatography , organic chemistry
The characteristics shown in the electrospray ionization/ion trap mass spectra of ring‐opened LI‐F antibiotics (cyclic depsihexapeptides with a 15‐guanidino‐3‐hydroxypentadecanoic group as a side‐chain) were examined. Collision‐induced dissociation (CID) MS of protonated molecules of the depsipeptides produced many fragment ions. Most of these fragment ions contained information for determining the amino acid sequences of antifungal antibiotics. The fragment ions were classified into six groups (b n ′ , B n ′ , B′ n ′ , β n ′ , y n and Y n ). According to MS 3 spectra, the B n ′ , B′ n and β n ions can be considered to be derived with a cleavage at each CO—NH in the peptide bonds of [MH −NH 3 ] + ,[MH −NH 3 −OH] + and [MH −NH 3 −2H 2 O] + , respectively, in ion trap MS. Losses of NH 3 and H 2 O from the amino acid residues of the depsipeptides in ion trap MS are likely to be smaller than those from the side‐chain. The measurements with electrospray ionization (ESI)/ion trap MS of depsipeptides with a side chain containing polar groups may provide useful information for structural determination. Copyright © 2001 John Wiley & Sons, Ltd.