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Iron Sucrose as MRI Contrast Agent in Ischemic Stroke Model
Author(s) -
Wu Shiman,
Zhang Hua,
Wang Jing,
Li Xiaoyan,
Gao Xinyi,
Fang Ziwei,
Qu Jianxun,
Wu Yue,
Ren Yan,
Rui Wenting,
Zhang Junhai,
Yao Zhenwei
Publication year - 2020
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.27109
Subject(s) - medicine , nuclear medicine , cerebral blood flow , gadolinium , middle cerebral artery , magnetic resonance angiography , perfusion , magnetic resonance imaging , stroke (engine) , radiology , occlusion , ischemia , chemistry , mechanical engineering , organic chemistry , engineering
Background Despite the growing concern about the safety of gadolinium‐based contrast agents (GBCAs), they are still the most commonly used. Ferumoxytol, as an off‐label alternative MRI contrast agent, cannot be administered by a rapid bolus for dynamic susceptibility contrast perfusion‐weighted imaging (DSC‐PWI). Purpose To assess the feasibility of iron sucrose (IS) as a contrast agent for MR angiography (MRA) and DSC‐PWI. Study Type Prospective animal model. Animal Model Thirty‐six normal rats (16 for MRA, 20 for biocompability tests) and 36 occlusion of the middle cerebral artery (MCAO) model rats. Field Strength/Sequence 3.0T; head and neck angiography, using a fast spoiled gradient‐recalled‐echo (FSPGR) sequence and DSC‐MRI using echo planar imaging(EPI) sequence. Assessment MRA was performed on normal rats to examine the signal‐to‐noise ratio (SNR) and contrast‐to‐noise ratio (CNR) of different doses of IS. DSC‐PWI was performed on MCAO rats at 0, 24, 48, and 72 hours postreperfusion to investigate the lesion detectability of IS. Arterial spin labeling (ASL) and DSC‐PWI enhanced by GBCAs were conducted on MCAO rats as controls. Statistical Tests Kruskal–Wallis test was used to compare qualitative assessment. One‐way analysis of variance (ANOVA) was used to compare the parametric data. Pearson's r values were evaluated between relative cerebral blood flow(rCBF)‐ASL, rCBF‐DSC IS , and rCBF obtained from DSC‐PWI enhanced by GBCA. Results The mean SNR and CNR of the common carotid artery at doses of 10 mg Fe/kg of IS were comparable with the standard dose of GBCAs (SNR: 68.04 ± 12.55 vs. 67.72 ± 14.66; CNR: 23.78 ± 7.21vs. 21.63 ± 6.83). In MCAO rat models, rCBF and relative cerebral blood volume (rCBV) of ipsilateral striatum declined (0.72 ± 0.14, 0.86 ± 0.11) with prolonged relative mean transit time (rMTT) and relative time‐to‐peak (rTTP) (1.27 ± 0.24, 1.07 ± 0.03) following occlusion. Hyperperfusion was observed in all rats at 48 and 72 hours postreperfusion, in 4/6 rats at 24 hours postreperfusion for IS‐mediated DSC‐PWI. Data Conclusion IS may be an effective contrast agent for both MRA and DSC‐PWI in ischemic stroke models. Level of Evidence 1 Technical Efficacy Stage 1 J. Magn. Reson. Imaging 2020;52:836–849.