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Probing demyelination and remyelination of the cuprizone mouse model using multimodality MRI
Author(s) -
Wang Nian,
Zhuang Jie,
Wei Hongjiang,
Dibb Russell,
Qi Yi,
Liu Chunlei
Publication year - 2019
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.26758
Subject(s) - diffusion mri , in vivo , ex vivo , corpus callosum , remyelination , medicine , nuclear medicine , fractional anisotropy , magnetic resonance imaging , white matter , multiple sclerosis , pathology , nuclear magnetic resonance , myelin , radiology , biology , physics , central nervous system , immunology , microbiology and biotechnology
Background Various studies by MRI exhibit that the corpus callosum (CC) is the most vulnerable to cuprizone administration, detecting the demyelination and remyelination process using different MRI parameters are, however, lacking. Purpose To investigate the sensitivity of multiparametric MRI both in vivo and ex vivo for demyelination and remyelination. Study Type Prospective. Animal Model A cuprizone mice model with an age‐matched control group ( n = 5), 4‐week cuprizone exposure group followed by 9‐week on a normal diet ( n = 6), and a 13‐week cuprizone exposure group ( n = 6). Field Strength/Sequence 3D gradient recalled echo, T 2 ‐weighted, and diffusion tensor imaging (DTI) at 7.0T and 9.4T. Assessment Quantification of DTI metrics, quantitative susceptibility mapping (QSM), and T 2 ‐weighted imaging intensity in major white matter bundles. Statistical Tests Nonparametric permutation tests were used with a cluster‐forming threshold as 3.09 (equivalent to P = 0.001), and the significant level as P = 0.05 with family‐wise correction. Results In vivo susceptibility values increased from –11.7 to –0.7 ppb ( P < 0.001) in CC and from –13.7 to –5.1 ppb ( P < 0.001) in the anterior commissure (AC) after the 13‐week cuprizone exposure. Ex vivo susceptibility values increased from –25.4 to 7.4 ppb ( P < 0.001) in CC and from –41.6 to –15.8 ppb ( P < 0.001) in AC. Susceptibility values showed high variations to demyelination for in vivo studies (94.0% in CC, 62.8% in AC). Susceptibility values exhibited higher variations than radial diffusivity for ex vivo studies (129.1% vs. 28.3% in CC, 62.0% vs. 25.0% in AC). In addition to the differential susceptibility variations in different white matter tracts, intraregional demyelination variation was also present not only in CC but also in the AC area by voxel‐based analysis. Data Conclusion QSM is sensitive to the demyelination process of cuprizone exposure, which can be a complementary technique to conventional T 2 ‐weighted images and DTI metrics. Level of Evidence : 2 Technical Efficacy Stage : 2 J. Magn. Reson. Imaging 2019;50:1852–1865.