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Acute kidney damage induced by low‐ and iso‐osmolar contrast media in rats: Comparison study with physiologic MRI and histologic‐gene examination
Author(s) -
Wu ChenJiang,
Bao MeiLing,
Wang Qing,
Wang XiaoNing,
Liu XiSheng,
Shi HaiBin,
Zhang YuDong
Publication year - 2017
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.25346
Subject(s) - medicine , pathology , contrast (vision) , kidney , magnetic resonance imaging , acute kidney injury , radiology , computer science , artificial intelligence
Purpose To investigate the physiopathological effects of low‐ and iso‐osmolar contrast media (CM) on renal function with physiologic MRI and histologic‐gene examination. Materials and Methods Forty‐eight rats underwent time‐course DWI and DCE‐MRI at 3.0 Tesla (T) before and 5–15 min after exposure of CM or saline (Iop.370: 370 mgI/mL iopromide; Iod.320: 320 mgI/mL iodixanol; Iod.270: 270 mgI/mL iodixanol; 4 gI/kg body weight). Intrarenal viscosity was reflected by apparent diffusion coefficient (ADC). Renal physiologies were evaluated by DCE‐derived glomerular filtration rate (GFR), renal blood flow (RBF), and renal blood volume (RBV). Potential acute kidney injury (AKI) was determined by histology and the expression of kidney injury molecule 1 (Kim‐1). Results Iop.370 mainly increased ADC in inner‐medulla (△ADC IM : 12.3 ± 11.1%; P < 0.001). Iod.320 and Iod.270 mainly decreased ADC in outer‐medulla (△ADC IM ; Iod.320: 16.8 ± 7.5%; Iod.270: 18.1 ± 9.5%; P < 0.001) and inner‐medulla (△ADC IM ; Iod.320: 28.4 ± 9.3%; Iod.270: 30.3 ± 6.3%; P < 0.001). GFR, RBF and RBV were significantly decreased by Iod.320 (△GFR: 45.5 ± 24.1%; △RBF: 44.6 ± 19.0%; △RBV: 35.2 ± 10.1%; P < 0.001) and Iod.270 (33.2 ± 19.0%; 38.1 ± 15.6%; 30.1 ± 10.1%; P < 0.001), while rarely changed by Iop.370 and saline. Formation of vacuoles and increase in Kim‐1 expression was prominently detected in group of Iod.320, while rarely in Iod.270 and Iop.370. Conclusion Iso‐osmolar iodixanol, given at high‐dose, produced prominent AKI in nonhydrated rats. This renal dysfunction could be assessed noninvasively by physiologic MRI. Level of Evidence: 1 J. Magn. Reson. Imaging 2017;45:291–302.