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Histological validation of cardiac magnetic resonance T 1 mapping for detecting diffuse myocardial fibrosis in diabetic rabbits
Author(s) -
Zeng Mu,
Zhang Nan,
He Yi,
Wen Zhaoying,
Wang Zhanhong,
Zhao Yike,
Greiser Andreas,
An Jing,
Zhang Tianjing,
Jing Bin,
Zhang Xin,
Fan Zhanming,
Li Debiao
Publication year - 2016
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.25341
Subject(s) - medicine , fibrosis , myocardial fibrosis , diabetes mellitus , magnetic resonance imaging , h&e stain , nuclear medicine , correlation , pathology , staining , cardiology , radiology , endocrinology , geometry , mathematics
Purpose To pathologically verify the correlation between native T 1 mapping, postcontrast T 1 mapping, and extracellular volume fraction (ECV) and myocardial diffuse fibrosis, as determined by collagen volume fraction (CVF). Materials and Methods Thirty New Zealand white rabbits were randomly divided into the control group ( n = 6), diabetes 3 months group ( n = 8), diabetes 6 months group ( n = 8), and diabetes 9 months group ( n = 8). All the rabbits underwent clinical 3.0T magnetic resonance (MR) examinations with pre‐ and postcontrast modified Look–Locker inversion recovery T 1 mapping. For the histological study, each rabbit was sacrificed after MR scanning, hematoxylin and eosin and Masson staining of the left ventricular myocardium were performed, and CVF was calculated. Pre‐ and postcontrast T 1 values and ECV were compared to CVF using Pearson's correlation coefficients. Results Two rabbits died in each diabetes group, thus each group included six rabbits. ECV calculated from pre‐ and postcontrast T 1 values showed a very strong correlation with CVF ( r = 0.876, P < 0.001), whereas postcontrast T 1 values exhibited a moderate correlation with CVF ( r = –0.564, P = 0.004). In contrast, precontrast T 1 values showed no correlation with CVF ( r = 0.311, P = 0.139). Conclusion ECV has a very strong correlation with pathological CVF, and can be used to assess the degree of diffuse myocardial fibrosis better than the postcontrast T 1 value. Precontrast T 1 value has no significant correlation with diffuse myocardial fibrosis. J. Magn. Reson. Imaging 2016;44:1179–1185.