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Magnetization transfer as a potential tool for the early detection of acute graft rejection after lung transplantation in mice
Author(s) -
Kenkel David,
Yamada Yoshito,
Weiger Markus,
Jungraithmayr Wolfgang,
Wurnig Moritz C.,
Boss Andreas
Publication year - 2016
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.25266
Subject(s) - medicine , lung , transplantation , magnetization transfer , lung transplantation , graft rejection , pathology , radiology , surgery , magnetic resonance imaging
Purpose To investigate the value of magnetization transfer (MT) measurements for assessment of acute rejection (AR) in a murine lung transplantation model. Materials and Methods Thirty mice including 15 C57BL/10 mice serving as donors and 15 C57BL/6 mice as recipients were examined in this study. MT imaging datasets were acquired on a 4.7 Tesla small animal MR scanner using a three‐dimensional zero echo time sequence with a Gaussian‐shaped MT prepulse with 1000° or 3000° flip angle and systematic variation of off‐resonance frequencies between 1000 and 15,000 Hz. After image acquisition, the images were qualitatively assessed, magnetization transfer ratio (MTR) values were calculated and lungs were taken for histologic examination including staining with hematoxylin/eosin, Masson's trichrome (collagen), and α‐smooth muscle (fibroproliferative tissue) staining. Results Lung transplantation was successfully performed in all 15 mice. All animals showed AR characterized by the presence of interstitial mononuclear cell infiltrates. There were significant differences of MTR in lungs with and without AR ( P = 0.007). With a flip angle of 1000°, the largest differences between the MTR of healthy lungs and lungs with AR were observed for an off‐resonance frequency of 10,000 Hz (difference MTR 1.80%) and 15,000 Hz (1.91%) and with a flip angle of 3000° at off‐resonance frequencies of 6000 Hz (1.37%) and 8000 Hz (1.70%). Conclusion MT measurements may provide a tool for the quantitative assessment of AR. J. Magn. Reson. Imaging 2016;44:1091–1098.