Premium
T 2 mapping at 7T MRI can quantitatively assess intramyocardial hemorrhage in rats with acute reperfused myocardial infarction in vivo
Author(s) -
Chen Wei,
Zhang Bing,
Xia Rui,
Zhang Ruzhi,
Xu Ziqian,
Chen Yushu,
Wang Chunhua,
Wang Lei,
Zheng Jie,
Gao Fabao
Publication year - 2016
Publication title -
journal of magnetic resonance imaging
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.563
H-Index - 160
eISSN - 1522-2586
pISSN - 1053-1807
DOI - 10.1002/jmri.25145
Subject(s) - medicine , magnetic resonance imaging , myocardial infarction , pathological , in vivo , nuclear medicine , infarction , cardiology , radiology , pathology , microbiology and biotechnology , biology
Purpose To investigate T 2 mapping at 7T magnetic resonance imaging (MRI) for the detection and quantification of reperfused intramyocardial hemorrhage (IMH) in a rat model. Materials and Methods Myocardial infarction (MI) was induced in 25 female rats. Rats were scanned at a 7T MRI 48 hours after reperfusion, using T 2 mapping and late gadolinium enhancement imaging. Gross sections of the left ventricular myocardium and corresponding hematoxylin and eosin staining were assessed for IMH. T 2 mapping images were matched with the gross sections. The IMH volume, expressed as a percentage of the left ventricular myocardial volume, of each heart determined by T 2 mapping was compared with that calculated by pathological gross examination. Results Six rats died. In all, 97 gross sections of the left ventricular myocardium from the 19 rats were matched with T 2 mapping images. IMH occurred pathologically in 68 gross sections, which was detected as hypointense cores by T 2 mapping in 63 images (93% sensitivity). Three T 2 mapping images with hypointense cores showed no hemorrhage on pathological sections (90% specificity). The positive and negative predictive values of hemorrhage on T 2 mapping were 95% and 84%, respectively. In terms of the IMH volume, there was no significant difference between T 2 mapping and pathological gross measurements (4.8 ± 2.4% vs. 5.3 ± 3.2%; P = 0.11). Conclusion T 2 mapping at 7T MRI can reliably detect and quantify IMH in rats in vivo. This may be useful as a noninvasive quantitative approach to investigating the mechanisms and evolution of MI and reperfusion injury. J. Magn. Reson. Imaging 2016;44:194–203.